Procedural Determination of Porcine Intestinal Alkaline Phosphatase (ALP) Activity Kinetics in Classical Nutrition Research

Abstract

The determination of porcine intestinal alkaline phosphatase (ALP) is very essential in biological and biomedical researches as it is a versatile enzyme that plays very important roles, especially in the liver/bile duct functions as well as in bone developments and turn over. Due to its versatile importance in biological studies it is imperative to clearly understand how ALP can be determined in biological tissues to better understand the biological implications in a study involving knowing its level in the tissue being investigated, such as that of porcine tissue or in DNA-related studies. This paper details the step-by-step procedures involved in measuring ALP using the western blotting procedure in a step-wise fashion that simply entails the followings: tissue preparation, gel electrophoresis, transfer, blotting and detection, respectively.